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Contenido

The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics andáspecifically for two-dimensional electrophoresis (2-DE). Detailed laboratory methods describe the process of sample fractionationáused toáreduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital informationáregarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set.áVolume 2 illuminates specific applications of sample preparation tools and fractionation strategies to study a variety of biological systems.

Invaluable for students of Biochemistry, Biomedicine, Biology and Genomicsand essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delves into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.

Detalles del artículo

• Páginas : 340
• Editorial : Springer
• Idioma : Inglés
• Fecha de Publicación : 01/01/2008
• ISBN: 9781617378799
• Serie: Methods in Molecular Biology 425
• Encuadernación : Rústica
• Nº Volúmenes : 1