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Contenido

This book presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics in general and for two-dimensional electrophoresis (2-DE) in particular. 2-DE, with its unique capacity to resolve thousands of proteins in a single run, is still a fundamental research tool for nearly all protein-related scientific projects. The methods described here in detail are not limited to 2-DE and can also be applied to other protein separation techniques. Contains step-by-step instructions that ensure successful results Provides tricks of the trade and notes on troubleshooting and avoiding known pitfalls

   INDICE: From the contents Application of fluorescence dye saturation labelling for differential proteome analysis of 1000 microdissected cells from pancreatic ductal adenocarcinoma precursor lesions.- Albumin and immunoglobulin depletion of human plasma.- Multi-component Immunoaffinity Subtraction and Reversed-phase Chromatography of Human Serum.- Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies.- Proteomics of Cerebrospinal Fluid: Methods for Sample Processing.- Sample Preparation of Bronchoalveolar Lavage Fluid.- Preparation of nasal secretions for proteome analysis.- Preparation of Urine Samples for Proteomic Analysis.- Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis.- Sample Preparation of Culture Medium from Madine-Darby Canine Kidney Cells.

Interés para ...

Biochemists, molecular and cellular biologists, and protein scientists

Detalles del artículo

• Páginas : 455
• Editorial : HUMANA PRESS
• Idioma : Inglés
• Fecha de Publicación : 01/08/2007
• ISBN: 9781603272094
• Serie: Methods in molecular biology 425
• Encuadernación : Cartoné
• Nº Volúmenes : 1
• País de Publicación : Reino Unido (INGLATERRA)